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p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))

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Entity

Name
CHRNA7
Namespace
hgnc
Namespace Version
20180906
Namespace URL
https://raw.githubusercontent.com/pharmacome/terminology/b46b65c3da259b6e86026514dfececab7c22a11b/external/hgnc-names.belns

Appears in Networks 1

Identification and Characterization of a G Protein-binding Cluster in alpha7 Nicotinic Acetylcholine Receptors v1.0.0

In-Edges 9

a(CHEBI:nifedipine) causesNoChange act(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))) View Subject | View Object

In α7345–348A nAChR expressing cells, nifedipine had no effect on the peak or the duration of the calcium transient (peak: 957.00% ΔF/Fθ ± 252.2%; AUC: 333.33% ΔF/Fθ2 × s ± 91.53%) relative to choline treatment alone (Fig. 5, A–C). The findings suggest that choline-induced calcium responses in PC12 cells involve the activity of VGCC (37, 38). PubMed:26088141

Appears in Networks:

a(MESH:"GP Antagonist-2A peptide") causesNoChange act(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))) View Subject | View Object

SP pretreatment did not significantly alter calcium peaks in α7345–348A nAChR expressing cells, showing a small (−31.24%) reduction in calcium responses relative to the α7345–348A baseline measure (p > 0.05). PubMed:26088141

Appears in Networks:

a(MESH:"xestospongin C") decreases act(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))) View Subject | View Object

Xestospongin C treatment did not impact the calcium peak (628.87% ΔF/Fθ ± 69.43%) or total calcium transient (AUC: 443.86% ΔF/Fθ2 × s ± 54.72%; + xestospongin C = 340.39% ΔF/Fθ2 × s ± 96.99%) in α7345–348A expressing cells (α7 to α7 + xestospongin C, p = 0.017; α7 to α7345–348A, p = 0.013; α7 to α7345-348A + xestospongin C, p = 0.017) (Fig. 7, B and C). The data underscores the inability of α7345–348A nAChRs to activate intracellular calcium via IP3Rs (18). PubMed:26088141

Appears in Networks:

a(MESH:Barium) causesNoChange act(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))) View Subject | View Object

As shown in Fig. 5, A–C, barium replacement had little to no effect on the peak and total calcium transient observed in α7 (peak: 1474.83% ΔF/Fθ ± 162.00%; AUC: 693.5% ΔF/Fθ2 × s ± 154.15%) and α7345–348A expressing cells (peak: 794% ΔF/Fθ ± 81.36%; AUC: 543.5% ΔF/Fθ2 × s ± 89.59%). PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7) hasVariant p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) View Subject | View Object

Appears in Networks:

complex(p(FPLX:"G_beta"), p(FPLX:"G_gamma"), p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))) hasComponent p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) View Subject | View Object

Appears in Networks:

complex(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")), p(HGNC:GNAQ)) hasComponent p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) View Subject | View Object

Appears in Networks:

composite(a(CHEBI:choline), p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))) hasComponent p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) View Subject | View Object

Appears in Networks:

g(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) causesNoChange p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) View Subject | View Object

Studies in N2a cells indicate that transfection with α7345–348A yields a similar α7 nAChR expression as the wild-type (α7) and supports the finding that mutation at the GPBC does not impact the synthesis of the nAChR in cells. PubMed:26088141

Appears in Networks:
Annotations
Experimental Factor Ontology (EFO)
Neuro-2a cell

Out-Edges 14

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) decreases bp(GO:"calcium-mediated signaling") View Subject | View Object

These findings in N2a cells are consistent with data from PC12 cells and suggest that α7345–348A nAChR expression impairs nAChR calcium signaling. PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) increases bp(HBP:"nAChR assembly") View Subject | View Object

The findings confirm that the mutation in α7345–348A does not interfere with the trafficking or expression of the nAChR. PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) increases p(HGNC:CHRNA7) View Subject | View Object

Western blot analysis confirms that transfection of α7 nAChRs augments total α7 subunit expression in PC12 cells, which express endogenous α7 nAChRs. Transfection with α7 (α7+) increased the immunoreactive α7 signal by over 60% from endogenous mock-transfected control cells, whereas transfection with the mutant α7345–348A increased the total α7 signal by 48% over the endogenous α7 from control cells (Fig. 3A). PubMed:26088141

Appears in Networks:
Annotations
MeSH
PC12 Cells

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) directlyDecreases complex(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")), p(HGNC:GNAQ)) View Subject | View Object

As shown in Fig. 3A, coupling between Gαq and α7 nAChRs was virtually abolished by expression of α7345–348A. A noticeable loss in Gαq (−62.18%) and Gβγ (−20.03%) expression within the α7 nAChR complex IP was seen in cells transfected with α7345–348A (Fig. 3A). PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) directlyDecreases complex(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")), p(HGNC:GNAQ)) View Subject | View Object

An increase in G protein association within the α7 complex was observed in α7+ cells (Gαq +16.71%; Gβγ +19.90%) (Fig. 3A). Similar findings in transfected N2a cells indicate a loss in G protein association within the α7 complex when α7345–348A nAChRs are expressed (Fig. 3B). PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) directlyDecreases complex(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")), p(HGNC:GNAQ)) View Subject | View Object

The data complements earlier findings on the ability of α7345–348A to function as a dominant negative blocker of G protein coupling in PC12 cells, and suggests that the GPBC directs nAChR association with Gαq and Gβγ. PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) directlyDecreases complex(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")), p(HGNC:GNAQ)) View Subject | View Object

Because α7345–348A- transfected cells did not show any responsiveness to SP, these findings suggest this receptor mutant is not functionally coupled to Gαq. PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) directlyDecreases complex(p(FPLX:"G_beta"), p(FPLX:"G_gamma"), p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))) View Subject | View Object

As shown in Fig. 3A, coupling between Gαq and α7 nAChRs was virtually abolished by expression of α7345–348A. A noticeable loss in Gαq (−62.18%) and Gβγ (−20.03%) expression within the α7 nAChR complex IP was seen in cells transfected with α7345–348A (Fig. 3A). PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) directlyDecreases complex(p(FPLX:"G_beta"), p(FPLX:"G_gamma"), p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))) View Subject | View Object

An increase in G protein association within the α7 complex was observed in α7+ cells (Gαq +16.71%; Gβγ +19.90%) (Fig. 3A). Similar findings in transfected N2a cells indicate a loss in G protein association within the α7 complex when α7345–348A nAChRs are expressed (Fig. 3B). PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) directlyDecreases complex(p(FPLX:"G_beta"), p(FPLX:"G_gamma"), p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A"))) View Subject | View Object

The data complements earlier findings on the ability of α7345–348A to function as a dominant negative blocker of G protein coupling in PC12 cells, and suggests that the GPBC directs nAChR association with Gαq and Gβγ. PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) decreases act(a(CHEBI:choline)) View Subject | View Object

PC12 cells transfected with α7345–348A showed a reduction in choline-mediated calcium responses. PubMed:26088141

Appears in Networks:
Annotations
MeSH
PC12 Cells

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) decreases a(CHEBI:"calcium(2+)") View Subject | View Object

PC12 cells transfected with α7345–348A showed a reduction in choline-mediated calcium responses. PubMed:26088141

Appears in Networks:
Annotations
MeSH
PC12 Cells

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) decreases act(a(CHEBI:"calcium(2+)", loc(GO:intracellular))) View Subject | View Object

Xestospongin C treatment did not impact the calcium peak (628.87% ΔF/Fθ ± 69.43%) or total calcium transient (AUC: 443.86% ΔF/Fθ2 × s ± 54.72%; + xestospongin C = 340.39% ΔF/Fθ2 × s ± 96.99%) in α7345–348A expressing cells (α7 to α7 + xestospongin C, p = 0.017; α7 to α7345–348A, p = 0.013; α7 to α7345-348A + xestospongin C, p = 0.017) (Fig. 7, B and C). The data underscores the inability of α7345–348A nAChRs to activate intracellular calcium via IP3Rs (18). PubMed:26088141

Appears in Networks:

p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")) decreases bp(MESH:"Pleckstrin Homology Domains") View Subject | View Object

In PC12 cells expressing α7345–348A nAChRs choline had a weak effect on PH-mCherry translocation relative to empty plasmid-transfected controls. Expression α7345–348A nAChRs was surprisingly associated with strong levels of PH-mCherry at the cell surface in the absence of drug treatment (Fig. 6B). PubMed:26088141

Appears in Networks:
Annotations
MeSH
Cytosol
MeSH
PC12 Cells

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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.