a(MESH:"xestospongin C")

Entity

Name

xestospongin C

Namespace

MeSH

Namespace Version

20181007

Namespace URL

https://raw.githubusercontent.com/pharmacome/terminology/01c9daa61012b37dd0a1bc962521ba51a15b38f1/external/mesh-names.belns

We confirmed the involvement of IP3Rs in choline-induced calcium transients at the GC of PC12 cells. Cells were preincubated with the IP3R blocker xestospongin C (1 μM) prior to imaging. As shown in Fig. 7, A–C, pretreatment with xestospongin C reduced the α7 nAChR calcium response peak by 46.82% in α7 cells (peak: 1308.43% ΔF/Fθ ± −238.13%; + xestospongin C = 695.80% ΔF/Fθ ± 101.46%). PubMed:26088141

We confirmed the involvement of IP3Rs in choline-induced calcium transients at the GC of PC12 cells. Cells were preincubated with the IP3R blocker xestospongin C (1 μM) prior to imaging. As shown in Fig. 7, A–C, pretreatment with xestospongin C reduced the α7 nAChR calcium response peak by 46.82% in α7 cells (peak: 1308.43% ΔF/Fθ ± −238.13%; + xestospongin C = 695.80% ΔF/Fθ ± 101.46%). PubMed:26088141

Xestospongin C treatment did not impact the calcium peak (628.87% ΔF/Fθ ± 69.43%) or total calcium transient (AUC: 443.86% ΔF/Fθ2 × s ± 54.72%; + xestospongin C = 340.39% ΔF/Fθ2 × s ± 96.99%) in α7345–348A expressing cells (α7 to α7 + xestospongin C, p = 0.017; α7 to α7345–348A, p = 0.013; α7 to α7345-348A + xestospongin C, p = 0.017) (Fig. 7, B and C). The data underscores the inability of α7345–348A nAChRs to activate intracellular calcium via IP3Rs (18). PubMed:26088141