complex(p(HGNC:CHRNA7, var("p.345A"), var("p.346A"), var("p.347A"), var("p.348A")), p(HGNC:GNAQ))
As shown in Fig. 3A, coupling between Gαq and α7 nAChRs was virtually abolished by expression of α7345–348A. A noticeable loss in Gαq (−62.18%) and Gβγ (−20.03%) expression within the α7 nAChR complex IP was seen in cells transfected with α7345–348A (Fig. 3A). PubMed:26088141
An increase in G protein association within the α7 complex was observed in α7+ cells (Gαq +16.71%; Gβγ +19.90%) (Fig. 3A). Similar findings in transfected N2a cells indicate a loss in G protein association within the α7 complex when α7345–348A nAChRs are expressed (Fig. 3B). PubMed:26088141
The data complements earlier findings on the ability of α7345–348A to function as a dominant negative blocker of G protein coupling in PC12 cells, and suggests that the GPBC directs nAChR association with Gαq and Gβγ. PubMed:26088141
Because α7345–348A- transfected cells did not show any responsiveness to SP, these findings suggest this receptor mutant is not functionally coupled to Gαq. PubMed:26088141
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.