PubMed: 26202471

Title
Predicting storage-dependent damage to red blood cells using nitrite oxidation kinetics, peroxiredoxin-2 oxidation, and hemoglobin and free heme measurements.
Journal
Transfusion
Volume
55
Issue
None
Pages
2967-78
Date
2015-12-01
Authors
Harper V | Marques MB | Oh JY | Patel RP | Stapley R

Evidence e73da9bcb7

More recently, we have also shown that free heme is also released during storage and may mediate further inflammation28

Evidence 0d233f8026

Prx-2 has emerged as the key antioxidant protein that protects RBCs against biologically relevant concentrations of H2O2 produced endogenously (via hemoglobin autoxidation) or exogenously by inflammatory cells25,26.

Evidence 18cfbd3317

Figures 2A-F show changes in oxyhemoglobin, methemoglobin and free heme in both the intraerythrocytic and supernatant fractions in both d7 and d35 RBC. Significant storage-dependent increases for all species in the cell-free fraction were observed, with no storage-dependent differences observed in the erythrocyte.

Evidence 403ee7b3c7

Figures 5A-5B, show that at d7, faster rates for nitrite oxidation in the lag phase were positively associated with higher concentrations of extracellular free heme at d7 and d35.

Evidence 5b5980bd11

We have also shown that older RBCs oxidize nitrite with faster rates compared to younger RBCs, which may account for decreased circulating nitrite levels in trauma patients receiving older RBCs14.

Evidence 594b342190

Figures 5C-5D show that d7 propagation rates for nitrite oxidation correlate with both d7 and d35 cell-free oxyhemoglobin.

Evidence f34d2d863d

Figures 4B-D shows that d7 Prx-2 oxidation correlates positively with nitrite oxidation kinetics in the lag phase (i.e. inversely with lag time, positively with lag rates, Figures 4B-C respectively).

Evidence ab0499f213

Figure 5E shows that d7 nitrite oxidation propagation rates also positively correlate with the extent of Prx-2 oxidation that occurred during storage (i.e. d35 – d7 Prx-2 oxidation in RBC).

Evidence 8999984a33

In other words, the faster rate of d7 nitrite oxidation predicted greater extents of oxidative damage incurred by the RBCs during storage.

Evidence a0dbc897a0

Figure 2G shows storage also resulted in a significant increase in Prx-2 oxidation.

Evidence 0c2a460f81

The higher the basal (d7) level of Prx-2 oxidation, the higher the level of Prx-2 oxidation in the same RBC after 35 days of storage.

Evidence 264d2b164a

Storage is known to result in increased hemolysis which in turn results in loss of NO-signaling, oxidative stress and inflammation post-transfusion.

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