composite(p(HBP:"Tau isoform F (441 aa)"), p(HGNC:STUB1))

Components

• p(HBP:"Tau isoform F (441 aa)")
• p(HGNC:STUB1)

CHIP co-expression led to a significant recovery in the area of mitochondria around the nucleus (19–26%). PubMed:25374103

Taken together, these data indicate that tau-overexpression leads to abnormal mitochondrial trafficking that can be rescued by CHIP-co-expression PubMed:25374103

Densitometry analysis of total tau levels of H4- cell lysates on SDS-PAGE showed a 2.0 to 2.5 fold lower levels of tau (4R-tau, tauC3, and K18ΔK280) with co-expression with CHIP, respectively (Fig. 1). PubMed:25374103

Co-transfection of CHIP with 4R-tau leads to a significant increase in Hsp70 levels, however this is not the case with wither tauC3 or K18DK280 constructs (Supplementary Fig. 2) suggesting that increased HSP70 protein levels are not necessary for the observed reduction in tau levels. PubMed:25374103

Tau overexpression increased active caspase 3 levels, and co-expression of CHIP reduced cleaved caspase 3 levels compared to tau expression alone (Fig. 3). PubMed:25374103

Indeed, the co-expression of CHIP caused a significant decrease tauC3 in vitro (Fig. 4). PubMed:25374103

CHIP co-expression led to a significant recovery in the area of mitochondria around the nucleus (19–26%). PubMed:25374103

Taken together, these data indicate that tau-overexpression leads to abnormal mitochondrial trafficking that can be rescued by CHIP-co-expression PubMed:25374103