complex(a(CHEBI:epibatidine), a(MESH:"alpha6beta2 nicotinic acetylcholine receptor"))

Components

• a(CHEBI:epibatidine)
• a(MESH:"alpha6beta2 nicotinic acetylcholine receptor")

This NACHO-mediated [3H]epibatidine binding to alpha6beta2 transfectants was also displaced by conotoxin MII (Figure 4E) PubMed:28445721

However, we did find that NACHO enhances [3H]epibatidine binding to cells transfected with alpha6beta2beta3 (Figure 4C) indicating that NACHO can enhance intracellular receptor assembly PubMed:28445721

Whereas this subunit combination is not competent to form a functional channel (Champtiaux et al., 2002; Dash et al., 2014; Kuryatov et al., 2000), NACHO still mediated partial receptor assembly as reflected by [3H]epibatidine binding (Figure 4D) PubMed:28445721

We first assayed expression of a6b2 or a3b2 receptors by binding the agonist, 125 I-labeled epibatidine (Fig. 1, A and B), to stably transfected a6FLAGb2HA cells and transiently trans- fected a3FLAGb2HA cells. PubMed:18174175