composite(p(HBP:"4R tau", var("p.Lys280del")), p(HGNC:STUB1))

Components

• p(HBP:"4R tau", var("p.Lys280del"))
• p(HGNC:STUB1)

CHIP co-expression led to a significant recovery in the area of mitochondria around the nucleus (19–26%). PubMed:25374103

Taken together, these data indicate that tau-overexpression leads to abnormal mitochondrial trafficking that can be rescued by CHIP-co-expression PubMed:25374103

Probing for phospho-tau with the 12E8 antibody in cell lysates from K18ΔK280 transfected cells reveals that tau phosphorylation is also decreased by CHIP overexpression (Fig. 1). PubMed:25374103

Densitometry analysis of total tau levels of H4- cell lysates on SDS-PAGE showed a 2.0 to 2.5 fold lower levels of tau (4R-tau, tauC3, and K18ΔK280) with co-expression with CHIP, respectively (Fig. 1). PubMed:25374103

After cyclohexamide treatment, we could still detect a significant decrease in tau levels in cells that were co-transfected with CHIP indicating that the reduction in tau levels due to CHIP is due to degradation and not to transcriptional down-regulation (Supplementary Fig. 3). PubMed:25374103

Probing for phospho-tau with the 12E8 antibody in cell lysates from K18ΔK280 transfected cells reveals that tau phosphorylation is also decreased by CHIP overexpression (Fig. 1). PubMed:25374103

Co-transfection of CHIP with 4R-tau leads to a significant increase in Hsp70 levels, however this is not the case with wither tauC3 or K18DK280 constructs (Supplementary Fig. 2) suggesting that increased HSP70 protein levels are not necessary for the observed reduction in tau levels. PubMed:25374103

CHIP co-expression led to a significant recovery in the area of mitochondria around the nucleus (19–26%). PubMed:25374103

Taken together, these data indicate that tau-overexpression leads to abnormal mitochondrial trafficking that can be rescued by CHIP-co-expression PubMed:25374103