Autoradiographic detection of dopamine D1 receptors by [125I]- SCH 23982 was fully displaceable by the agonist SKF-77434.
Overall, no changes were observed in dopamine D1 receptor binding in the brain regions examined following social isolation and ⁄ or antalarmin treatment under these conditions
antalarmin increased dopamine D2 ⁄ 3 binding in the CPu (+7%, P < 0.001; Fig. 3C) and olfactory tubercle (OT; +15%, P 1⁄4 0.006; Fig. 3D) of group-housed rats.
Antalar- min treatment reversed this effect and returned dopamine D2 ⁄ 3 receptor density levels back to those of the vehicle-treated GH rats.
Furthermore a significant interaction was found between housing and treatment in both the central nucleus of the amygdala (CeA; F1,139 1⁄4 11.560, P < 0.001; Fig. 3F) and basolateral amygdala (BLA; F1,137 1⁄4 7.616, P 1⁄4 0.007; Fig. 3E).Thus, isolation caused an up-regulation in dopamine D2 ⁄ 3 receptors in these regions, an effect that was reversed by antalarmin treatment (but only reached statistical significance in the CeA).
Treatment with antalarmin had no impact upon the isolation-induced alterations of BDNF expression.
As was the case with BDNF expression, treatment with antalarmin had no impact upon the isolation-induced alterations in TrkB expression.
Interestingly however, antalarmin treatment of GH rats caused a down-regulation of TrkB mRNA in the LC ()15%, P 1⁄4 0.012) and Pir ()26%, P 1⁄4 0.013) whilst increases were observed in the hippocampus (CA1–3; +36%, P 1⁄4 0.042) and RSc (+53%, P < 0.001).
Isolation rearing caused an up-regulation of dopamine D2 ⁄ 3 receptors in both the core (+20%; F1,144 1⁄4 31.176, P < 0.001; Fig. 3A) and shell (+25%; F1,148 1⁄4 35.233, P < 0.001; Fig. 3B) of the NAcc.
A significant decrease in BDNF mRNA expression resulting from ISO was found in the BLA ()25%; F1,102 1⁄4 77.937, P < 0.001), nucleus of the horizontal limb of the diagonal band (HB )75%; F1,67 1⁄4 259.106, P < 0.001) and dentate gyrus (DG )85%; F1,101 1⁄4 984.958,
whilst isolation increased BDNF mRNA expression in the retrosplenial cortex
ISO resulted in a decrease in TrkB mRNA expression in the cingulate cortex (Cg1–3; F1,124 1⁄4 7.582, P 1⁄4 0.014) and piriform cortex (Pir; F1,119 1⁄4 3.869
whereas an increase was observed in isolates compared to GH in the hippocampus (+38%; F1,59 1⁄4 14.131, P < 0.001), DG (+32%; F1,56 1⁄4 8.398, P 1⁄4 0.005) and RSc (+32%; F1,56 1⁄4 6.830, P 1⁄4 0.011).
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.