PubMed: 22764226

Title
Acute administration of L-DOPA induces changes in methylation metabolites, reduced protein phosphatase 2A methylation, and hyperphosphorylation of Tau protein in mouse brain.
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Volume
32
Issue
None
Pages
9173-81
Date
2012-07-04
Authors
Arning E | Bottiglieri T | Nunbhakdi-Craig V | Sontag E | Sontag JM | Wasek B

Evidence ecc7739bbc

Reductions in PP2A methylation were associated with a concomitant increase in each brain region of p-Tau at the PHF-1 epitope (Fig. 6A,B).

Evidence ab9b16966e

Incubation of human SH-SY5Y neuroblastoma cells for 2 h with L-dopa induced a dose-dependent decrease in both soluble and insoluble methylated PP2A C subunit levels, and concomitant accumulation of demethylated PP2A enzymes

Evidence c73d88e2c6

Significantly, we found similar effects in human dopaminergic neurons (Fig. 2F). Treatment of neurons with L-Dopa induced a 40–49% decrease in methylated PP2A and concomitant 168–179% increase in endogenous p-Tau (PHF-1) levels (Fig. 2G).

Evidence 5889979b83

Folate starvation in the presence of L-dopa resulted in a 200–210% increase in demethylated PP2A levels, and 235–250% increase in p-Tau levels in the dopaminergic neurons, relative to untreated controls maintained in NF medium.

Evidence 855d91182e

Here, we found that administration of L-dopa led to a significant 5-fold increase in plasma tHcy concentrations, when compared to saline treated mice (Table 1). This L-dopa induced increase in plasma tHcy was significantly greater in mice that had been reared on either low folate (LF) or FD diets (Table 1).

About

BEL Commons is developed and maintained in an academic capacity by Charles Tapley Hoyt and Daniel Domingo-Fernández at the Fraunhofer SCAI Department of Bioinformatics with support from the IMI project, AETIONOMY. It is built on top of PyBEL, an open source project. Please feel free to contact us here to give us feedback or report any issues. Also, see our Publishing Notes and Data Protection information.

If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.