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PubMed: 12551948

Title
14-3-3 connects glycogen synthase kinase-3 beta to tau within a brain microtubule-associated tau phosphorylation complex.
Journal
The Journal of biological chemistry
Volume
278
Issue
None
Pages
12722-8
Date
2003-04-11
Authors
Cafferty PW | Han D | Qureshi HY | Yuan Z | Lin R | Agarwal-Mawal A | Paudel HK

Evidence 8711fb8e67
JSON

The results suggest that the amino-terminal fragment suppresses neurite outgrowth by competing for plasma membrane binding.

act(complex(GO:"plasma membrane")) negativeCorrelation p(INTERPRO:"MAP2/Tau projection") View Subject | View Object

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Experimental Factor Ontology (EFO)
PC12

p(INTERPRO:"MAP2/Tau projection") decreases bp(GO:"neuron projection development") View Subject | View Object

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Experimental Factor Ontology (EFO)
PC12

p(INTERPRO:"MAP2/Tau projection") negativeCorrelation act(complex(GO:"plasma membrane")) View Subject | View Object

Appears in Networks:
Annotations
Experimental Factor Ontology (EFO)
PC12

Evidence e70b777356
JSON

These observations suggested that 14-3-3zeta may be bound to GSK3Beta and/or tau within brain microtubules and may be a component of tau phosphorylation complex.Therefore, 14-3-3 must be the central molecule that holds tau and GSK3Beta within the complex. Indeed, FLAG-tau co-immunoprecipitated with HA-GSK3Beta from cells overexpressing FLAG-tau and HA-GSK3Beta only when these cells also overexpressed Xpress-14-3-3zeta (Fig. 6A, lanes 8 and 9), indicating that GSK3Beta associates with tau only in the presence of 14-3-3zeta. As discussed above, 14-3-3zeta binds to tau (36) and GSK3Beta (Fig. 5) directly. Taken together, these observations indicated that 14-3-3zeta connects GSK3Beta to tau in vivo.

p(HGNC:MAPT) association p(HGNC:YWHAZ) View Subject | View Object

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Experimental Factor Ontology (EFO)
HEK293

p(HGNC:GSK3B) association p(HGNC:YWHAZ) View Subject | View Object

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Experimental Factor Ontology (EFO)
HEK293

p(HGNC:YWHAZ) association p(HGNC:MAPT) View Subject | View Object

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Experimental Factor Ontology (EFO)
HEK293

p(HGNC:YWHAZ) association p(HGNC:GSK3B) View Subject | View Object

Appears in Networks:
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Experimental Factor Ontology (EFO)
HEK293

Evidence 80fbb9fb6e
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In cells that were co-transfected with fixed amounts of FLAG-tau and HA-GSK3Beta but different amounts of Xpress-14-3-3zeta, FLAG-tau phosphorylation increased progressively with the increase in the amount of Xpress-14-3-3zeta (lanes 7–9). This increase was evident not only by an increased immunoreactivity against all tau phosphorylation-sensitive antibodies tested but also by a retarded mobility of FLAG-tau on the SDS gel, a characteristic feature of hyperphosphorylated tau (2, 3). Thus, 14-3-3zeta profoundly stimulated GSK3Beta-catalyzed tau phosphorylation in vivo.

act(p(HGNC:GSK3B)) increases p(HGNC:MAPT, pmod(Ph)) View Subject | View Object

Appears in Networks:
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Experimental Factor Ontology (EFO)
HEK293

p(HGNC:YWHAZ) increases act(p(HGNC:GSK3B)) View Subject | View Object

Appears in Networks:
Annotations
Experimental Factor Ontology (EFO)
HEK293

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