p(HBP:"Tau oligomers", var("p.Lys280del")) increases act(p(HGNC:CASP3))

View Subject View Object

PubMed:28528849

To address this question, we first applied the oligomers directly after the purification of the protein eluting from the Butyl FF 16/ 10 column (without buffer exchange; 1, 5, and 10 mM) to SHSY5Y cells and observed a variety of toxic effects, including pronounced reduction in the cell viability (by MTT assay, Fig. 3A), increase in apoptotic cells (by Hoechst staining, Supplementary Fig. 4A), loss of mitochondrial membrane potential (by JC1 assay, Supplementary Fig. 4B), caspase 3/7 activation (Supplementary Fig. 4C-D), and cytochrome-c release (Supplementary Fig. 4), within 5 hours of incubation.

Related Edges 5

• p(HBP:"Tau oligomers", var("p.Lys280del")) increases bp(GO:"cell death")
• p(HBP:"Tau oligomers", var("p.Lys280del")) increases bp(GO:"apoptotic process")
• p(HBP:"Tau oligomers", var("p.Lys280del")) increases bp(GO:"negative regulation of mitochondrial membrane potential")
• p(HBP:"Tau oligomers", var("p.Lys280del")) increases act(p(HGNC:CASP7))
• p(HBP:"Tau oligomers", var("p.Lys280del")) increases sec(p(HGNC:CYCS))

Experimental Factor Ontology (EFO)

SK-N-SH

BEL Commons is developed and maintained in an academic capacity by Charles Tapley Hoyt and Daniel Domingo-Fernández at the Fraunhofer SCAI Department of Bioinformatics with support from the IMI project, AETIONOMY. It is built on top of PyBEL, an open source project. Please feel free to contact us here to give us feedback or report any issues. Also, see our Publishing Notes and Data Protection information.

If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.

---