p(HBP:"Tau oligomers", var("p.Lys280del")) increases bp(GO:"cell death")

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PubMed:28528849

To address this question, we first applied the oligomers directly after the purification of the protein eluting from the Butyl FF 16/ 10 column (without buffer exchange; 1, 5, and 10 mM) to SHSY5Y cells and observed a variety of toxic effects, including pronounced reduction in the cell viability (by MTT assay, Fig. 3A), increase in apoptotic cells (by Hoechst staining, Supplementary Fig. 4A), loss of mitochondrial membrane potential (by JC1 assay, Supplementary Fig. 4B), caspase 3/7 activation (Supplementary Fig. 4C-D), and cytochrome-c release (Supplementary Fig. 4), within 5 hours of incubation.

Related Edges 5

• p(HBP:"Tau oligomers", var("p.Lys280del")) increases bp(GO:"apoptotic process")
• p(HBP:"Tau oligomers", var("p.Lys280del")) increases bp(GO:"negative regulation of mitochondrial membrane potential")
• p(HBP:"Tau oligomers", var("p.Lys280del")) increases act(p(HGNC:CASP3))
• p(HBP:"Tau oligomers", var("p.Lys280del")) increases act(p(HGNC:CASP7))
• p(HBP:"Tau oligomers", var("p.Lys280del")) increases sec(p(HGNC:CYCS))

Experimental Factor Ontology (EFO)

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