Title

The effect of CRH and its inhibitor, antalarmin, on in vitro growth of preantral mouse follicles, early embryo development, and steroidogenesis.

Journal

Endocrinology

Volume

154

Issue

None

Pages

222-31

Date

2013-01-01

Authors

Anagnostou E | Chrousos GP | Dinopoulou V | Drakakis P | Loutradis D | Makrigiannakis A | Mavrogianni D | Partsinevelos GA

At all embryo stages, significant differences were noted between the CRH 10􏰁7 and control groups as well as be- tween the CRH 10􏰁7 and CRH 10􏰁7/antalarmin 10􏰁6 groups, with the rates being lower in the former.

With regard to the morula/blastocyst stage, the rates in the CRH 10􏰁7 group were lower compared with the control (P 􏰄 0.003) and lower compared with the CRH 10􏰁7/antalarmin 10􏰁6 group (P 􏰃 0.001) (Table 2).

Interestingly, the addition of a 10-fold excess of the CRH-R1 antagonist, antalarmin, overcame the negative effect of CRH on estradiol release (d 5, P 􏰄 0.009; d 7, P 􏰄 0.010; d 9, P 􏰃 0.001; and d 11, P 􏰄 0.002), indicating that the suppressive effect of CRH was receptor mediated (Fig. 1).

The addition of the CRH-R1 an- tagonist antalarmin in a 10-fold higher concentration compared with that of CRH overcame the effect of CRH on beta􏰇-hCG release (d 11 beta􏰇-hCG 185.6 􏰅 6.087 mIU/ml, P 􏰃 0.001) indicating that the suppressive effect of CRH was recep- tor mediated (Fig. 3).

The addition of a 10-fold excess of the CRH-R1 antagonist antalarmin reversed the negative ef- fect of CRH on progesterone release (d 11 progesterone 0.712 􏰅 0.004 ng/ml, P 􏰃 0.001), suggesting that the suppressive effect of CRH was receptor mediated (Fig. 2).

The addition of the CRH-R1 an- tagonist antalarmin in a 10-fold higher concentration compared with that of CRH overcame the effect of CRH on 􏰇-hCG release (d 11 beta􏰇-hCG 185.6 􏰅 6.087 mIU/ml, P 􏰃 0.001) indicating that the suppressive effect of CRH was recep- tor mediated (Fig. 3).

In the CRH 10􏰁7 mol/liter group, the amount of beta􏰇-hCG measured in the culture medium was elevated but to a lesser extent compared with the control group (d 11 􏰇-hCG 6.05 􏰅 0.077 mIU/ml, P 􏰃 0.001).

Notably, CRH did not affect the amount of progesterone released into the incubation medium up to d 9, whereas it suppressed pro- gesterone levels on d 11 (d 11 progesterone 0.030 􏰅 0.000 ng/ml, P 􏰃 0.001).

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