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Nicotinic acid is synthesized from quinolinic acid through pyridine nucleotide cycle, and is controlled by quinolinate phosphoribosyltransferase (QPRT)

N-methyl-Δ1-pyrrolinium is synthesized from arginine and/ or ornithine, and is controlled by putrescine N-methyl transferase (PMT), which is the key enzyme in diverting metabolism towards the biosynthesis of nicotine and others alkaloids [12, 13].

Experiments involving RNA-mediated gene silencing with transgenic Nicotiana plants have shown that a decrease in PMT expression levels can lead to low nicotine content

Down-regulation of PMT expression led to elevation of anatabine level in transgenic tobacco root lines and leaf tissues of regenerated tobacco plants

Nicotine is synthesized through condensation of two transitory compounds, N-methyl-Δ1-pyrrolinium and nicotinic acid [5, 12].

Anatabine is synthesized from nicotinic acid, and QPRT is alike an enzyme of key importance in regulating anatabine biosynthesis in tobacco (Fig. 1)

To shed further light on pathway regulation, the question was asked about whether alteration of nicotine and anatabine content would affect the expression of other key enzyme, namely QPRT, which is a key enzyme in the biosynthesis of another precursor of nicotine, and also the key enzyme of anatabine (Fig. 1).

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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.