Next, we tested whether exogenous IL-13 can also enhance epithelial repair. Injured monolayers of 1HAEo- cells were treated with different concentrations of IL-13 (1–100 ng/ml). As shown in Figure 2B, addition of IL-13 at 10, 30, and 100 ng/ml significantly stimulated epithelial repair (‡P , 0.01).
To understand the effects of cytokines on epithelial cells in asthma, we have investigated the effects of interleukin (IL)-4, IL-13, and interferon (IFN)-γ on barrier function and wound healing in Calu-3 human lung epithelial cells. IL-4 and IL-13 treatment of Calu-3 cells grown on Transwell filters resulted in a 70–75% decrease in barrier function as assessed by electrophysiological and [14C]mannitol flux measurements.
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