Name
colorectal cancer
Annospace Keyword
Disease
Annospace Name
Disease Ontology (DO)
Annospace Version
20170511
Annospace URL
https://arty.scai.fraunhofer.de/artifactory/bel/annotation/disease/disease-20170511.belanno

Sample Edges 5

g(HGNC:FCGR1CP) association bp(MESHPP:"Antibody-Dependent Cell Cytotoxicity")

SNPs in genes encoding Fcγ receptors are functionally relevant to cetuximab-mediated ADCC in colorectal cancer PubMed:26817995

p(HGNC:IGF1) increases p(HGNC:IRS1, pmod(Ph, Tyr))

We found that IGF-1 stimulation enhanced tyrosine phosphorylation of two proteins, beta-catenin and insulin-receptor substrate 1, which formed a complex with E-cadherin. Tyrosine phosphorylation of beta-catenin was accompanied by rapid (<1 min) dissociation from E-cadherin at the plasma membrane, followed by relocation to the cellular cytoplasm. PubMed:11035789

p(HGNC:IGF1) increases p(HGNC:CTNNB1)

IGF-1 also enhanced the stability of beta-catenin protein. PubMed:11035789

Annotations
NCBI Taxonomy Ids
9606
Experimental Factor Ontology (EFO)
Caco-2
Experimental Factor Ontology (EFO)
DLD-1 cell
Disease Ontology (DO)
colorectal cancer
MeSH
Plasma

p(HGNC:IGF1) increases act(p(HGNC:CTNNB1), ma(tscript))

To investigate whether IGF-1 also enhanced transactivation potential, a reporter gene assay was performed in HEK293 cells. We used a reporter construct incorporating multimeric Lef/Tcf promoter sequences upstream of a luciferase reporter (TOPFLASH) and a control construct containing mutated promoter sequences (FOPFLASH, ref. 21). We cotransfected TOPFLASH or FOPFLASH, ?-catenin, and human wild-type Tcf-4 wild-type expression constructs into 293 cells. Optimized luciferase assays showed a basal level of TOP/FOP activity of between 4:1 and 20:1. Forty-eight hours after transfection, serum-starved 293 cells were treated with IGF-1 or insulin for 3 or 6 h. These factors alone failed to alter basal reporter activity (Fig. 6 b). A 16-h pretreatment of the transfectants with LiCl had a dramatic effect on reporter activity, with significant enhancement at 50 mM LiCl (P < 0.01 by Tukey's test for comparison with activity in the absence of LiCl). We saw a further increase in reporter activity when we added 50 ng/ml (6.5 nM) IGF-1 to transfectants pretreated with LiCl at 10 mM (P < 0.01 after 6 h of IGF-1 treatment) and at 50 mM (P < 0.01 after 3 h of IGF-1 treatment and P < 0.001 after 6 h). PubMed:11035789

Annotations
NCBI Taxonomy Ids
9606
Experimental Factor Ontology (EFO)
Caco-2
Experimental Factor Ontology (EFO)
DLD-1 cell
Disease Ontology (DO)
colorectal cancer
MeSH
Plasma

p(HGNC:IGF1) increases p(HGNC:CTNNB1, pmod(Ph, Tyr))

We found that IGF-1 stimulation enhanced tyrosine phosphorylation of two proteins, beta-catenin and insulin-receptor substrate 1, which formed a complex with E-cadherin. Tyrosine phosphorylation of beta-catenin was accompanied by rapid (<1 min) dissociation from E-cadherin at the plasma membrane, followed by relocation to the cellular cytoplasm. PubMed:11035789

About

BEL Commons is developed and maintained in an academic capacity by Charles Tapley Hoyt and Daniel Domingo-Fernández at the Fraunhofer SCAI Department of Bioinformatics with support from the IMI project, AETIONOMY. It is built on top of the open source project, PyBEL. Please feel free to contact us here to give us feedback or report any issues.